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Huvet, Arnaud; Beguel, Jean-philippe; Pereira Cavaleiro, Nathalia; Thomas, Yoann; Quillien, Virgile; Boudry, Pierre; Alunno-bruscia, Marianne; Fabioux, Caroline. |
Feeding strategies and digestive capacities can have important implications for variation in energetic pathways associated with ecological and economically important traits, such as growth or reproduction in bivalve species. Here, we investigated the role of amylase in the digestive processes of Crassostrea gigas, using in vivo RNA interference. This approach also allowed us to investigate the relationship between energy intake by feeding and gametogenesis in oysters. Double-stranded (ds)RNA designed to target the two α-amylase genes A and B was injected in vivo into the visceral mass of oysters at two doses. These treatments caused significant reductions in mean mRNA levels of the amylase genes: −50.7% and −59% mRNA A, and −71.9% and −70.6% mRNA B in 15... |
Tipo: Text |
Palavras-chave: Alpha-Amylase; DsRNA; Gametogenesis; Marine bivalve; Dynamic energy budget. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00271/38239/36422.pdf |
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